Beta1 Integrin, Human, mAb BV7 – 20 µg - HM2033-20UG
Quantity
20 µg
Catalog #
HM2033-20UG
133,00 €
The monoclonal antibody BV7 recognizes human ß1-integrin. Beta-1 integrin is a ubiquitously
expressed ~89 kDa type I transmembrane protein functioning as receptor when heterodimerized with
one alpha subunit. It belongs to the integrin beta chain family consisting of four different genes,
encoding multiple β-integrins via alternative splicing. Ligand-recognition depends on the composition
of the heterodimer: either collagen, fibronectin, VCAM1, laminin, cytotactin, osteopontin, epiligrin,
thrombospondin and CSPG4 can bind to the integrin-complex. Beta-1 integrins recognize the
sequence R-G-D in a wide array of ligands. Isoform beta-1B interferes with isoform beta-1A resulting
in a dominant negative effect on cell adhesion and migration (in vitro). In case of HIV-1 infection, the
interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma
lesions. When associated with α7, β1-integrin regulates cell adhesion and laminin matrix deposition.
BV7 is active on HT-29 colon carcinoma cells and on HCCP-2998 tumor cells. It is involved in
promoting endothelial cell motility and angiogenesis. Furthermore, β1-integrin plays a mechanistic
adhesive role during telophase, and is required for the successful completion of cytokinesis.
Upon activation integrins in general, including β1-integrin, are known to exhibit global structural
rearrangements and exposure of ligand binding sites. β1-integrin modulation is of importance in tissue
repair and regeneration. In cultured primary hippocampal neurons, astrocytes and tissues, cell surface
expression of amyloid beta fibrils (key hallmark of Alzheimer’s disease) selectively co-localized with
β1-integrin. Preincubation of cells with antibodies against β1-integrin, as well as α1-integrin, greatly
enhanced amyloid beta-induced apoptosis, indicating a protective role for integrins in apoptosis.
The monoclonal antibody BV7 does not recognize α5β1 complex and not the cytoplasmic part of the
β1-subunit. Monoclonal antibody BV7 is active on HT-29 colon carcinoma cells and on HCCP-2998
tumor cells. BV7 binds to several other tumor cells (MG3 osteosarcoma, A375 melanoma, MHCC-
1410 and Lovo colon carcinoma) but does not affect adhesion to endothelial cells.
expressed ~89 kDa type I transmembrane protein functioning as receptor when heterodimerized with
one alpha subunit. It belongs to the integrin beta chain family consisting of four different genes,
encoding multiple β-integrins via alternative splicing. Ligand-recognition depends on the composition
of the heterodimer: either collagen, fibronectin, VCAM1, laminin, cytotactin, osteopontin, epiligrin,
thrombospondin and CSPG4 can bind to the integrin-complex. Beta-1 integrins recognize the
sequence R-G-D in a wide array of ligands. Isoform beta-1B interferes with isoform beta-1A resulting
in a dominant negative effect on cell adhesion and migration (in vitro). In case of HIV-1 infection, the
interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma
lesions. When associated with α7, β1-integrin regulates cell adhesion and laminin matrix deposition.
BV7 is active on HT-29 colon carcinoma cells and on HCCP-2998 tumor cells. It is involved in
promoting endothelial cell motility and angiogenesis. Furthermore, β1-integrin plays a mechanistic
adhesive role during telophase, and is required for the successful completion of cytokinesis.
Upon activation integrins in general, including β1-integrin, are known to exhibit global structural
rearrangements and exposure of ligand binding sites. β1-integrin modulation is of importance in tissue
repair and regeneration. In cultured primary hippocampal neurons, astrocytes and tissues, cell surface
expression of amyloid beta fibrils (key hallmark of Alzheimer’s disease) selectively co-localized with
β1-integrin. Preincubation of cells with antibodies against β1-integrin, as well as α1-integrin, greatly
enhanced amyloid beta-induced apoptosis, indicating a protective role for integrins in apoptosis.
The monoclonal antibody BV7 does not recognize α5β1 complex and not the cytoplasmic part of the
β1-subunit. Monoclonal antibody BV7 is active on HT-29 colon carcinoma cells and on HCCP-2998
tumor cells. BV7 binds to several other tumor cells (MG3 osteosarcoma, A375 melanoma, MHCC-
1410 and Lovo colon carcinoma) but does not affect adhesion to endothelial cells.
| Datasheet URL | https://www.hycultbiotech.com/wp-content/uploads/2022/06/coa-tds_hm2033-100ug.pdf |
|---|---|
| Quantity | 20ug |
| Quantity | 20 µg |
| Species | human |
| Alias | CD29, fibronectin receptor subunit beta,-integrin VLA-4 subunit beta |
| Application | Flow cytometry, Functional studies, Immuno assays, Immuno precipitation, Western blot |
| Precautions | For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product. |
| References | 1. Martin-Padura, I et al; A novel mechanism of colon carcinoma cell adhesion to the endothelium
triggered by ß1 integrin chain. J Biol Chem 1994, 269: 6124 2. Palmieri, D et al;Trimer carboxyl propeptide of collagen I produced by mature osteoblasts is chemotactic for endothelial cells. J Biol Chem 2000, 275: 32658 3. Martel, V et al; Talin controls exit of the integrin α5β1 from an early compartment of the secretory pathway. J Cell Sci 2000, 113: 1951 4. Hofmann, G et al; HERG K+ channels activation during β1 integrin-mediated adhesion to fibronectin induces an up-regulation of αvβ3 integrin in the preosteoclastic lekemia cell line FLG29.1. J Biol Chem 2001, 276: 4923 5. Bozzo, C et al; Involvement of β1 integrin in βAP-induced apoptosis in human neuroblastoma cells. Mol Cell Neurosci 2004, 25: 1 |
| Disease | Autoimmunity, Neurological disorders |
| Application: | Flow cytometry Functional studies Immuno assays Immuno precipitation Western blot |
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