MPO, Human, ELISA kit - HK324-01

Name: MPO, Human, ELISA kit - HK324-01
Brand: Hycult Biotech
SKU: HK324-01
Price: 713.00 EUR
Availability: InStock

Quantity

1 x 96 det.

Catalog #

HK324-01

713,00 €

Details

Advanced insights with the MPO Human ELISA kit The MPO human ELISA offers precise MPO quantification in human samples. It measures MPO, a key glycoprotein, with unparalleled accuracy. MPO is essential in myeloid cells. It is found in neutrophils' azurophilic granules. MPO is crucial for the body's defense. It aids in phagocytic lysis and produces HOCl, a strong oxidant. Activated neutrophils release MPO rapidly. MPO's role is vast. It is linked to diseases like atherosclerosis and lung cancer. It is also connected to Alzheimer's and multiple sclerosis. In Wegener's disease, it is targeted by autoimmune antibodies. MPO deficiency, once overlooked, is now a key research area. The classical MPO assay is an enzymatic assay for activity of MPO. This classical MPO assay is hampered by the presence of inhibitory compounds in tissue homogenates and plasma. In this type of assays spiking often gives unreliable results. The human MPO ELISA is not influenced by inhibitors of the enzyme activity. Perform curve fitting with Assayfit Pro with the online ELISA calculation sheets. Click here for the terms for use and distribution.

Weitere Informationen

Weitere Informationen
Datasheet URL	https://www.hycultbiotech.com/wp-content/uploads/2023/05/SDS-HK324.pdf
Quantity	1 x 96 det.
Quantity	1 x 96 det.
Standard range	0.4 to 100 ng/ml
Detection level	0.4 ng/ml
Working volume	100 µl/well
Species	Human
Cross reactivity	Baboon - No, Horse - No, Mouse - No, Pig - No, Rabbit - No, Rat - No, Sheep - No
Application	The human MPO ELISA kit is to be used for the in vitro quantitative determination of human MPO in plasma, sputum and cell culture supernatant samples.
Principle	The human MPO ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 3½ hours. The efficient format of a plate with twelve disposable 8-well strips allows free choice of batch size for the assay. Samples and standards are incubated in microtiter wells coated with antibodies recognizing human MPO. Biotinylated tracer antibody will bind to captured human MPO. Streptavidin-peroxidase conjugate will bind to the biotinylated tracer antibody. Streptavidin-peroxidase conjugate will react with the substrate, tetramethylbenzidine (TMB). The enzyme reaction is stopped by the addition of oxalic acid. The absorbance at 450 nm is measured with a spectrophotometer. A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the human MPO standards (log). The human MPO concentration of samples, which are run concurrently with the standards, can be determined from the standard curve.
Storage and stability	Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least six months.
Precautions
References	1. Leclercq A et al; Involvement of intraplaque hemorrhage in atherothombosis evolution via neutrophil protease enrichment. J Leukoc Biol 2007, 82: 1420 2. Martin-Ventura J et al ; Low plasma levels of HSP70 in patients with carotid atherosclerosis are associated with increased levels of proteolytic markers of neutrophil activation. Atherosclerosis 2007, 194: 334 3. Puklo, M et al; Analysis of neutrophil-derived antimicrobial peptides in gingival crevicular fluid suggests importance of cathelicidin LL-37 in the innate immune response against periodontogenic bacteria. Oral Microbiol Immunol 2008, 23: 328 4. Haan de, J et al; Postshock intervention with high-lipid enteral nutrition reduces inflammation and tissue damage. Ann Surg 2008, 248: 842
Disease	Cardiology and metabolism, Pulmonology

Applications
Application assays:	The human MPO ELISA kit is to be used for the in vitro quantitative determination of human MPO in plasma, sputum and cell culture supernatant samples.
Principle:	The human MPO ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 3½ hours. The efficient format of a plate with twelve disposable 8-well strips allows free choice of batch size for the assay. Samples and standards are incubated in microtiter wells coated with antibodies recognizing human MPO. Biotinylated tracer antibody will bind to captured human MPO. Streptavidin-peroxidase conjugate will bind to the biotinylated tracer antibody. Streptavidin-peroxidase conjugate will react with the substrate, tetramethylbenzidine (TMB). The enzyme reaction is stopped by the addition of oxalic acid. The absorbance at 450 nm is measured with a spectrophotometer. A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the human MPO standards (log). The human MPO concentration of samples, which are run concurrently with the standards, can be determined from the standard curve.